Shared lab equipment for molecular applications

The mission of the SEFS Shared Genetics Lab is to provide a facility that supports cutting-edge molecular research with environmental applications. The lab provides training and assistance with genetic research projects, including the processing and analysis of a variety of plant, fungal, and animal sample types. The lab specializes in processing environmental DNA (e.g., water, air, and soil samples) and other non-invasive samples (e.g., scat, hair, saliva) characterized by low-copy, degraded DNA. Separate rooms for pre- and post-PCR reduce contamination risk for these samples. The laboratory is equipped with new technologies, such as a droplet digital PCR machine, to detect and analyze very low levels of DNA.

​This laboratory is open for use by UW students and researchers interested in using the equipment for genetic research. Equipment use and contract work are also available to external researchers. First time users should contact the lab manager for details.

Available Equipment

Sample processing
  • Tube-format and plate-format centrifuges
  • Freezone freeze-dryer/lyophilizer
  • TissueLyser II
  • Sample shaker
  • Dry baths
  • Fume hood
  • Sample vortexers
  • PCR hood workstation
  • pH meter
  • Heated magnetic stir block
  • Magnetic stands for plate and tube-based SPRI bead cleaning​
Amplification and analysis
  • QX200 Digital droplet PCR (ddPCR) generator, reader, plate sealers, and laptop with analysis software
  • CFX96 Real-time PCR optical reaction thermal cycler
  • Four C1000 96-well PCR thermal cyclers
  • T100 Deep-well PCR thermal cycler
  • Qubit fluorometer
  • NanoDrop spectrophotometer
  • Gel electrophoresis running rigs
  • Gel EZ Doc Gel electrophoresis imager with imaging plates and laptop with analysis software
  • GeneMapper 6 license
  • CLC Workbench license ​​
Other
  • Mini autoclave
  • Single and multi-channel pipettes (0.5µl-1000µl)
  • -80°C freezers
  • -20°C freezers
  • 5°C refrigerator
  • Various consumables 


​Rates

Consumables

Consumable
Unit
Internal rate per unit
External rate per unit
​0-20 µL filtered pipette tips
​1 box of 96 tips
​$10.00
$11.55
​20-200 µL filtered pipette tips
​1 box of 96 tips
​$10.00
$11.55
​100-1000 µL filtered pipette tips
​1 box of 96 tips
$11.00
$12.71
​96-well PCR plate (price dependent on plate type)
​1 plate
$2-$5
$2.32-$5.78
​1.5 ml or 2 ml microcentrifuge tubes
​1 tube
$0.21
$0.24
​Qubit tubes
​1 tube
$0.20
$0.23
​Qubit reagents
1 ml
$0.66
$0.76
Foil plate seal
1 seal
$1.00
$1.16

ddPCR usage

Lab supplies used
Internal Rate
External rate
Equipment and reader oil only
$20 per 8 wells
$23.12 per 8 wells
All consumables (except PCR plates and foil seals, which are available separately)
$40 per 8 wells
$46.24 per 8 wells

Contract work

​Contract work is offered by the lab and is priced on a case-by-case basis. Contact the lab manager (Ellie Reese, [email protected]) for contract work inquiries.