The laboratory consists of three DNA processing areas. It is important to keep the flow of the lab unidirectional (moving from field sample to final PCR product) due to the low quality/low quantity nature of the DNA in noninvasive samples. Even low amounts of PCR product (amplicons) can contaminate samples and cause false findings.
There are two adjacent rooms that will be used to extract DNA from the samples. These rooms are equipped with a centrifuge, thermomixer, stir plate, balance, pH meter, and vortexers. Chemical processes are used to extract the DNA from the source, sometimes in commercially available kits.
This room is used to prepare PCR reactions after DNA is isolated from the sample. The room is equipped with a PCR workstation that provides a clean environment for prepping the reaction and the ability to UV radiate the work area to destroy any left behind DNA. This room also contains instruments used to prepare the oil droplets and reaction used for the Droplet Digital PCR machine. A -20 degree freezer holds reagents for pre-PCR work.
The thermocyclers, Droplet Digital PCR Reader, and agarose gel station/Gel Doc EZ Imager are in the PCR room. It is important to keep all lab supplies, samples, and lab coats separated between this room and the Pre-PCR and Extraction rooms due to the potential of amplicon contamination. This is the final room for processing samples where DNA isolated from the sample is amplified for detection. This room also contains a freezer for high DNA products.